Extract the bursin from the bursa fabricius of chichens and ducks through the ultrafilter with loooda filter . ( the bursin of chickens - cbs . the bursin of ducks - dbs ) determine the major constitution by high - performance liquid chromatography ( hplc ) and thin - layer chromatography ( tlc ) . it is proved that there is bursin ( bs ) in two extractions . caculate the quantitation of bursin in the extraction of the bursa fabricius of chickens and ducks . it can be found through tlc that the quantitation of cbs in the extraction of the bursa fabricius of chickens is 1 . 436mg / ml and the quantitation of dbs in the extraction of the bursa fabricius of ducks is 4 . 035mg / ml . the result obtained through hplcis that the quantitation of cbs is 1 . 199mg / ml and dbs is 3 . 316mg / ml 采用超滤法从正常鸡、鸭法氏囊组织中提取分子量小于1000da的物质，分别以薄层层析法和高压液相色谱法检测其主要成分，证明两种超滤液中均有法氏囊活性肽(囊素三肽， bursin ， bs )的存在，分别计算鸡、鸭法氏囊提取物中的囊素三肽的含量。薄层层析方法求得鸡法氏囊提取物中的bs含量为1 . 436mg / ml ，鸭法氏囊提取物中的bs含量为4 . 035mg / ml ；高压液相色谱的方法求得鸡法氏囊提取物中的贴含量为1 . 199mg / ml ，鸭法氏囊提取物中bs含量为3 . 316mg / ml 。
